Research Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Guangming, Shenzhen ?
Email: [email protected]
Research
Our lab is lcoated in the Medical Building on?Shenzhen Campus of Sun Yat-sen University. We are dedicated to uncovering the molecular mechanisms of disease-relevant, challenging protein targets through an integrated approach combining biophysical and biochemical methods. Guided by insights into protein structure and dynamics, our goal is to develop innovative therapeutic and diagnostic strategies. Our current research focuses on:
1.KRAS mutations in tumors and genetic disorders?– We focus on both hotspot and rare KRAS mutations, investigating novel tumor-suppressive mechanisms involving RAS proteins and developing targeted therapeutic strategies.
2.Neurotransmitter:sodium symporters (NSSs)?– We study the structural mechanisms of drug actions?on NSSs and explore the potential role of NSS in modulating amyloidiosis associated with neurodegenerative diseases.
3.GPCR ligand discovery?– We are developing innovative screening strategies to identify allosteric ligands targeting G protein-coupled receptors.
4.Targeted Protein Degradation?– We investigate the dysregulation of proteolytic pathways under pathological conditions and explore their implications for disease progression and therapeutic opportunities.
Our lab actively engages in cutting-edge collaborations and is committed to translating biological research into clinical applications to address unmet medical needs.
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Education and Accademic Experience
BSc., Chemistry, Xi’an Jiaotong University; MS, Chemistry, Renmin University of China; Dr. rer. nat., Biology, University of G?ttingen/Max-Planck-Institute for Biophysical Chemistry (current MPI-Multidisciplinary Sciences);
Postdoc., Department of biochemistry and biophysics, School of Medicine, the University of North Carolina at Chape Hill;
Associate Research Scientist, Columbia University Irving Medical Center
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Recent Pubilcations
[1] Ajit Prakash, Zijian Li, Venkat Chirasani, Juhi Rasquinha, Natalie Hewitt, Garrett Hubbard, Guowei Yin, Aspen Hawkins, Luca Montore, Henrik Dohlman, Sharon Campbell, Molecular and functional profiling of Gαi as an intracellular pH sensor, Nat Commun, 2025, 16:3468.
[2] Qi Liang , Haiyue Zuo, Tian Yang, Jiatai Yin, Xiaomin Huang, Jing Wang, Qian Li, Guowei Yin*, Xinfeng Zhao*, Discovery of dual-target ligands binding to beta2-adrenoceptor and cysteinyl-leukotriene receptor for the potential treatment of asthma from natural products derived DNA-encoded library, Eur. J. Med. Chem., 2022, 233:114212.
[3]?Guowei Yin*,?Jing Huang, Johnny Petela, Hongmei Jiang, Yuetong Zhang, Siqi Gong, Jiaxin Wu, Bei Liu, Jianyou Shi, Yijun Gao, Targeting small GTPases: emerging grasps on previously untamable targets, pioneered by KRAS, Signal Transduct Target Ther., 2023, 8:212.
[4] Guowei Yin*, Guohua Lv, Jerry Zhang, Hongmei Jiang, Tianqi Lai, Yushan Yang, Yong Ren, Jing Wang, Chenju Yi, Hao Chen, Yun Huang, Chaoni Xiao, Early-stage structure-based drug discovery for small GTPases by NMR spectroscopy, Pharmacol. Ther., 2022, 236:108110.
[5] Qian Li#, Guowei Yin#, Jing Wang, Linkang Li, Qi Liang, Xue Zhao, Yuanyuan Chen, Xiaohui Zheng, Xinfeng Zhao, An emerging paradigm to develop analytical methods based on immobilized transmembrane proteins and its applications in drug discovery, Trends in Analytical Chemistry, 2022, 157: 116728.
[6] Kevin Knight, Soumadwip Ghosh, Sharon Campbell, Tyler Lefevre, Reid Olsen, Alan Smrcka, Natalie Valentin, Guowei Yin, Nagarajan Vaidehi, and Henrik G. Dohlman, A Universal allosteric mechanism for G protein activation, Mol. Cell, 2021, 81:1-13.
[7] Vinay Nair, Guowei Yin, Jerry Zhang, John Hancock, Sharon Campbell, Alemayehu A Gorfe, Monoubiquitination of KRAS at lysine104 and lysine147 Modulates Its dynamics and interaction with partner proteins, J. Phys. Chem. B, 2021, 125: 4681-4691.
[8] Guowei Yin#, Jerry Zhang#, Vinay Nair, Vinh Truong, Angelo Chaia, Johnny Petela, Joseph Harrison, Alemayehu Gorfe, Sharon Campbell, KRAS ubiquitination at lysine 104 retains exchange factor regulation by dynamically modulating the conformation of the interface, iScience, 2020, 23:101448.
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